{"id":6643,"date":"2023-09-07T10:22:19","date_gmt":"2023-09-07T10:22:19","guid":{"rendered":"https:\/\/www.nrc.gov.lk\/?page_id=6643"},"modified":"2023-09-07T10:22:19","modified_gmt":"2023-09-07T10:22:19","slug":"transcriptome-analysis-of-mycobacteria-in-serum-exosomes-of-latent-tuberculosis-patients-for-candidate-biomarker-identification","status":"publish","type":"page","link":"https:\/\/www.nrc.gov.lk\/index.php\/transcriptome-analysis-of-mycobacteria-in-serum-exosomes-of-latent-tuberculosis-patients-for-candidate-biomarker-identification\/","title":{"rendered":"Transcriptome analysis of mycobacteria in serum exosomes of latent tuberculosis patients for candidate biomarker identification."},"content":{"rendered":"

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Transcriptome analysis of mycobacteria in serum exosomes of latent tuberculosis patients for candidate biomarker identification.<\/h2>\n<\/div>\n
<\/div><\/div><\/div><\/div><\/div><\/section>

NRC Grant No:<\/text><\/b>  22-011<\/text><\/p>

Research Institute:  <\/text><\/b>University of Colombo<\/text><\/p>

Area of Research:<\/text><\/strong> Platelet Inhibition<\/text><\/p>

Status:<\/text><\/strong>  Ongoing<\/text><\/p><\/div><\/div><\/div><\/div><\/div>

\n \"\"\n <\/figure>
\n

Principal Investigator\r\n<\/h2>

Prof. D.N. Magana-Arachchi
\nNational Institute of Fundamental Studies
\ndhammika.ma@nifs.ac.lk<\/p>\n<\/div>\n <\/article><\/div><\/div>

\n \"\"\n <\/figure>
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<\/h2><\/div>\n <\/article><\/div><\/div><\/div><\/div><\/section>
<\/p>\n

Summary<\/h2>\n

Tuberculosis (TB) is a global public health emergency responsible for millions of deaths. One-third of the world population is infected with latent TB, a silent TB carrier capable of progressing into active TB in the future. Currently available diagnostic tools are lengthy, time-consuming, and inefficient in detecting TBinfected young children and less immune individuals. With the current situation, TB diagnosis, treatment, and prevention services will be interrupted, resulting in additional TB cases and extra deaths. This urges the need for an accurate, rapid, and cost-effective diagnostic tool for TB diagnosis. Therefore, advanced molecular methods could be used for TB diagnosis purposes. More specifically bacterial biomarkers for disease diagnosis as the biomarkers derived from hosts show individual variations and much more research in the application. Therefore, much attention is driven towards pathogen gene expression using exosomes (small particles carrying biological molecules in the cell) for biomarker identification. Limited studies are conducted both globally and locally, warranting more future studies to end TB soon and prevent unnecessary deaths.<\/p>\n

\n<\/div><\/div><\/div>

<\/p>\n

Objectives<\/h2>\n

1. A preliminary study to detect Mtb RNA in serum exosomes using Pt8\/Pt9 primers and five Mtb and host genes (based on previous the literature) namely,
\u2022 Mtb genes: GlnA1, DnaK, GarA, FAS and Ag85a
\u2022 Host genes: CXCL10, GBP1, XPO4, CCL24, ABCA1 in ATB, LTBI and HCs using Real Time-
PCR.
2. To investigate the mycobacterial gene expression using serum exosomes of LTB patients and ATB
patients using RNA sequencing .3. To validate the top 15 differentially expressed genes obtained from RNA sequencing using RT-PCR for latent tuberculosis candidate biomarker identification<\/p>\n

\n<\/div><\/div><\/div><\/div><\/div><\/section>

<\/p>\n

Major Equipment Facilitated by Grant<\/h2>\n
Contents updating…<\/div>\n

\n<\/div><\/div><\/div><\/div><\/div><\/section>

<\/p>\n

\nDesignation<\/strong><\/p>\n

Principal Investigator (PI)<\/p>\n

Deputy Principal Investigator (DPI)<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

Co- investigator<\/p>\n

\n<\/div><\/div><\/div>

<\/p>\n

\nName<\/strong><\/p>\n

Dr. Meththika Vithanage<\/p>\n

Dr. B.C.L. Athapattu<\/p>\n

Prof. Ajith de Alwis<\/p>\n

Dr. Mahinsasa Narayana<\/p>\n

Dr. A. K. Karunarathna<\/p>\n

Prof. Ranji Ranjithan<\/p>\n

Dr. Mahinsasa Narayana<\/p>\n

Prof. Taeho Lee<\/p>\n

Dr. Maneesha P. Ginige<\/p>\n

\n<\/div><\/div><\/div>

<\/p>\n

\nInstitute<\/b><\/p>\n

University of Sri Jayewardenepura<\/p>\n

The Open University of Sri Lanka<\/p>\n

University of Moratuwa<\/p>\n

University of Moratuwa<\/p>\n

University of Peradeniya<\/p>\n

Waste Management Authority, Western Province<\/p>\n

North Carolina State University, USA<\/p>\n

Pusan National University Korea<\/p>\n

CSIRO, Australia<\/p>\n

\n<\/div><\/div><\/div><\/div><\/div><\/section>

<\/div><\/div><\/div><\/div><\/div><\/section>
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